IP injected human MSC rapidly formed aggregates with mouse macrophages and B220+ lymphocytes and attached to the walls of the peritoneal cavity. Gastroenterology 114, 433440 (1998). (A) MSCs were labeled with DiR dye and given by three different ways. PubMed Central Method. Available at: http://www.cdc.gov/ibd/ (Accessed: 7th April 2016) (2014). Afterwards, the samples were incubated with Ki-67 (1:200; Santa Cruz) or FOXP3 (1:100; Antibodies-Online, Aachen, Germany) overnight at 4C. East-West gradient in the incidence of inflammatory bowel disease in Europe: the ECCO-EpiCom inception cohort. In order to explore the possible mechanisms of these alterations, oxidative stress and inflammation levels in the mouse brain were examined. . Liu, T. et al. CBSKL2015Z01 and CBSKL2015Z12), PLA 12th Five-Year Program (BSW11J013) and National Science and Technology Major Project (No. Yu-Lung Chang, Huei-Yu Lo, Chin-Kan Chan, Xing-hua Pan, Qing-qing Li, Guang-ping Ruan, Hongsheng Yang, Rui Feng, Shenghong Zhang, H. R. Wardill, C. E. M. de Mooij, N. M. A. Blijlevens, Suze A. Jansen, Edward E. S. Nieuwenhuis, Caroline A. Lindemans, Scientific Reports Intraperitoneal injection | Detailed Pedia (B) Quantification of % Ki-67+ cells/crypt, the IP and IA>IV>DSS control, n=6 mice/group; n=3 for the control group. Wang, N. et al. One study utilized IP injections to study pain in the abdomen after a hysterectomy when administering anesthetic continuously vs patient-controlled. CAS PubMed Central mRNA expression of inflammatory mediators such as TNF-, IL-6, IL-1b, IL-10 and IFN- in the colon were measured by real time PCR. Zhu, H. et al. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Wang, M., Liang, C., Hu, H. et al. Our work used marrow-derived MSCs but not adipose-derived ones. We even tried 1108 cell/mouse in IP and AI group and no immediate death was occurred. We also evaluated serum TSG-6 in three MSCs administration route. Disclaimer. . Macrophage Reverse Cholesterol Transport | Circulation Little is known about neuronal cell death in mouse models of diabetic retinopathy. Article PubMed Department of Digestive Diseases, State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xian, 710032, China, Min Wang,Cong Liang,Hao Hu,Lin Zhou,Bing Xu,Xin Wang,Ying Han,Yongzhan Nie,Jie Liang&Kaichun Wu, Department of Gastroenterology, Xian Childrens Hospital, 710006, China, Department of Respiratory and Gastroenterology, Second Peoples Hospital, Xian, 710005, China, Department of Gastroenterology, PLA No.5 Hospital, Yinchuan, 750004, China, You can also search for this author in Intraperitoneal Versus Interpleural Morphine or Bupivacaine for Pain In anal injection group, same amount of cells were suspended in 200L diluted Matrigel in PBS (1:20) and the cell suspension was instilled into colonic lumen by a syringe with flexible catheter 4cm in length and 2mm in diameter, according to the previous study64. Mol Ther 18, 13651372, doi: 10.1038/mt.2010.54 (2010). seem to have found the key drug of MSCs in the treatment of colitis, TSG-647. Thus, we have succeeded to produce patient-like tumor colonization of the peritoneal cavity and hepatic metastasis with the human EAC cell line OE19 by intraperitoneal injection in SCID mice. Zhang, B. et al. Histopathological alterations in the mouse brain were detected after SWCNTs exposure. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. The accessibility of the therapeutic cells to their expectable target site is strongly dependent on the route of administration used. TSG-6 might be a major anti-inflammatory mechanism of MSCs, since not only in colitis model, the therapeutic effect of TSG-6 remotely secreted by MSCs were also identified in many other disease model46,48,49,50. The physiologic characteristic of the peritoneal cavity not only helps remove toxic metabolites from the body, but also provides a useful portal of entry in the body for several pharmacological agents. PubMed prepared Figure 4. Admittedly, there may be an overlook of MSCs existence due to the tissue selection of frozen section and sensitivity of fluorescence imaging, the therapeutic differentiation of MSCs in our study were not clearly observed. prepared Figure 5. In contrast, GFP+ cells were showed in the lumen for AI group and lamina propria for IV group (Fig. . Truncal blocks and neuraxial . Understanding when intraperitoneal injections can be utilized and in what applications is beneficial to advance current drug delivery methods and provide avenues for further research. Ko, I. K. et al. Lu, Z., Wang, J., Wientjes, M. G. & Au, J. L. Intraperitoneal therapy for peritoneal cancer. After intraperitoneal injections, 30% of the female mice died, compared with 3% of the male. The provided examples show a variety of uses for intraperitoneal injections in animals for in vitro studies. After centrifuge, the cells were certificated and transferred to MSC expansion culture medium consisting of a-minimum essential medium (a-MEM; Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 100g/mL penicillin/streptomycin. Intraperitoneal injection is widely used in rodents as a route of drug administration. Kushnir, C. L., Fleury, A. C., Silver, D. F. & Spirtos, N. M. Intraperitoneal Catheter Placement: The Hammock Technique. Use " or 1" needles to avoid penetrating abdominal organs. -Lactalbumin is an example of a "retired" self-protein because its expression in normal . In order to compare the difference of MSCs colonization among the three delivery routes, a nontoxic NIR tracer DiR was introduced to label MSCs. Journal of immunology (Baltimore, Md. [2], There has been some debate on whether intraperitoneal injections are the best route of administration for experimental animal studies. When searching "intraperitoneal injections" a majority of the results relate to the use of IP injections in murine or rat models in order to study the effect of some drug or chemical. The labeled cells were twice with warm fresh medium at 1500rpm for 5min to ensure complete removal of any unbound dye. MSCs has been well characterized of producing a range of modulatory cytokine, which inspires the designation of these cells as injury drugstore44. Lee, R. H. et al. Article Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone. Statistical significance was determined using one-way ANOVAs or Students t tests. WO2023056606A1 - Valproate (vpa) compounds for use in treating supply of IGF-I to adolescent and adult animals or humans is not known. Colonic mRNA expression of inflammatory mediators such as TNF-, IL-6, IL-1, IL-10 and IFN- were analyzed by real time-PCR in order to evaluate the local inflammatory environment (Fig). Histological evaluation was completed in a double-blind fashion by two independent pathologist. 81421003 and No. Intraperitoneal (IP) Injection in Rats and Mice SOP Page 4 of 6 Mouse Rat 6. Like pharmacological treatment, the cell dosing might also be an important issue for MSCs therapeutic success21. One study attempted to diagnose hepatic hydrothorax with the use of injecting Sonazoid intraperitoneally. It is estimated that as many as 1.4million Americans and 2.4million Europeans are suffering from these diseases1,2. Influence of Aggregation and Route of Injection on the - PLOS . Severity of DSS-induced colitis was also assessed using a score system by two independent pathologists. Inflammatory Scores were calculated using a score system66: Epithelium (E): 0, normal morphology; 1, loss of goblet cells; 2, loss of goblet cells in large areas; 3, loss of crypts; 4, loss of crypts in large areas. Antunes, M. A. et al. In colonic MLNs, similar results were observed, the MSCs administration induced more FoxP3+ cells accumulation than the control, but the inter-group differences were not evident (Supplementary Figure S8). Ulcerative colitis (UC) and Crohns disease (CD) are most common conditions in IBD. Intratumoral injection of hydrogel-embedded nanoparticles enhances & Gratwohl, A. Article Proper Method for Intraperitoneal Injections 2020 May 9;12(5):439. doi: 10.3390/pharmaceutics12050439. Therapeutic strategies should be based on a sound and thorough understanding of the disease mechanisms, if possible, however the causes of IBD are still unclear6. Normal mouse IgG was used as a substitute for the primary antibody as negative control. Rodent (large / mouse) oral (intragastric), intravascular administration (tail vein injection, intraperitoneal injection, intramuscular injection and subcutaneous injection) Intragastric administration and oral taking of non-rodent (dog / monkey) solution and intravenous injection of extremities. Overall, this section provides a few examples of the effects and uses of intraperitoneal injections in human patients. of the needle exposed to assure 4-5mm injection depth. We used SCID mice over nude mice for the metastatic survival model because the survival effects were more reproducibly obtained in SCID mice than in nude . Intraperitoneal (IP) route of drug administration in laboratory animals is a common practice in many in vivo studies of disease models. Google Scholar. Figure 1. Our findings showed that the GFP+ MSCs were migrated to the inflamed colon, even passed through the whole intestinal wall and reached the luminal side. MathSciNet In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. Twenty-five Kunming mice were exposed to Fe 3 O 4-nanoparticles via intraperitoneal injection daily for 1 week at doses of 0, 5, 10, 20, and 40 mg/kg. MSCs were isolated from C57BL/6 mice bone marrow as previously reported62. 81371615), the Stake Key Laboratory Open Program of China (No. [6] In 1968, melatonin was delivered to rats intraperitoneally in order to study how brain serotonin would be affected in the midbrain. IP, intraperitoneal injection; IV, intravenous injection; AI anal injection; n = 6-8 for treatment group, n = 3 for DSS control. For some severe cases, IBD may lead to the life-threaten complications5. Opioids can produce peripheral analgesic effects by activation of opioid receptors on sensory nerves. Not only in animal studies, some clinical trials using MSCs to treat osteogensis imperfect (OI) in GvHD, also showed less than 1% cell were detected in target organ18,19,20. All the tissue sections in this study were scan and analyzed with Virtual slide scanning system (VS120-S, Olympus). All reactions were performed in triplicated. Caplan, A. I. Article ADS GFP+ cells were detected at the inflamed colon. Intraperitoneal Route of Drug Administration: Should it Be Used in Just mentioned above, immune cells and MSCs interaction in aggregates promotes TSG-6 secretion and contributed to the colitis recovery. This inconsistency may attributed to different MSCs types. Available at: http://ctep.cancer.gov/highlights/20060105_ovarian.htm (Accessed: 7th April 2016) (2006). PubMed Cancers | Free Full-Text | Targeted Vaccination against Human The IP technique is an easy technique to master. Mice were under intensive observation for 12 days. Most importantly, IP was found to have highest serum TSG-6 at both 48h and 72h and showed statistical significance when comparing with AI and DSS control at 72h. These findings, to some extent, may help to understand why IP is better than AI and IV. Ahmadian Kia, N. et al. Another example is applying hammock catheter placement technique60. Monitor chest movements to make sure the animal is doing ok. On the mouse you want to make your IP injection in the lower right or left quadrant of abdomen trying to avoid hitting bladder, liver, or other internal organs. (A) The colonic mRNA expression of TNF- and IL-10 was analyzed by RT-PCR; (B) cytokine contents in colonic protein extracts were determined by ELISA; n=6 mice/group; n=3 for the control group (C) Serum TSG-6 level in different groups measured by ELISA. Exposure to BHT through inhalation, dermal, and oral routes is harmful. The .gov means its official. X.W., Y.H., Y.N. was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity . Kean, T. J., Lin, P., Caplan, A. I. Google Scholar. The latter is . Easier to inject that intravenous. [citation needed], In humans, the method is widely used to administer chemotherapy drugs to treat some cancers, particularly ovarian cancer. The complications associated with IP injection are major concern. Intraperitoneal injection - Wikipedia Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy. The AI groups exhibited 9.5% and IV group showed 6.7% FoxP3+ cells, respectively (Fig. Our results indicated that the intraperitoneal injection is the best delivery way for MSCs, which showed better mucosa recovery and higher cell engraftment at inflamed colon. Curr Opin Gastroenterol 30, 8496, doi: 10.1097/MOG.0000000000000031 (2014). Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Tyndall, A. In addition, IP showed more intense Ki-67 stain in bottom of the crypt, which suggested more active mucosa repair proliferation (Fig. In IV group, most of MSCs were trapped in the lung, liver and spleen, while the cell that immigrated to the colon was not too much. analyzed the data, J.L. Comparison of colonic FoxP3 expression 7 days after MSCs injection. The increasing evident indicated that cell-cell contact between MSCs and immune cells may also be of importance52,53,54. 5C). Intraperitoneal injection of a substance in the mouse. Dosage: For intravenous (preferred), intracardial, intrapleural, or intraperitoneal injection. Levy, O. et al. Intraperitoneal injections as an alternative method for micro-CT The number of therapeutic cells that can migrate and colonize at the injury site is a decisive prerequisite for the success of cytotherapy. The transplanted lymphocytes can produce immunoglobulin (Ig), respond to antigens, and survive for months in this chimeric model (hu-PBL SCID). Burisch, J. et al. Evid Based Complement Alternat Med. Clinical and experimental immunology 116, 238245 (1999). Radiation Protection of Polydatin Against Radon Exposure Injury of Tests were read by an independent observer (Cong Liang) and scored 05 as shown by the color indicators provided by the manufacturer. In this study, the intraperitoneal injection was demonstrated as the superior way for MSCs administration in the setting of colitis amelioration. Google Scholar. 2021. The site is secure. Intraperitoneal Route of Drug Administration: Should it Be Used in Respir Res 15, 118, doi: 10.1186/s12931-014-0118-x (2014). Some research showed exogenous CD34 stem cells migration towards the inflamed colon and differentiation into endothelial cells40. Indeed, Mariana et al. Injection into egg 1 month 20 doses 1 dose 1 month 1 month Once 3.0 mL/kg 5 mL/kg 10 mL/kg 2.5 mL/kg 1 mL/kg O.^L/g Well tolerated Well tolerated Well tolerated Well tolerated Well tolerated Less mortality 1 \Jg Increase in mortality, decreased activity during righting reflex, running time, visual discrimination, and olfactory aversion test By systemic evaluation of epithelium and lymphocyte infiltration, the IP injection group showed the relatively lower severity score compared with AI group (P=0.034) and IV (P=0.002), respectively. Comparison of molecular profiles of human mesenchymal stem cells derived from bone marrow, umbilical cord blood, placenta and adipose tissue. Ankrum, J. The overall therapeutic factors, MSCs migration and targeting as well as local immunomodulatory cytokines and FoxP3+ cells infiltration were analyzed. volume6, Articlenumber:30696 (2016) In our study, we tracked the GFP+ MSCs colon colonization, but our results also showed the short stay of MSCs after injection. The three groups can decrease the mortality rate compared with PBS control, however, the IP injection showed the highest survival rate of 87.5% (P=0.0021vs. Control, Fig. . Because it allows administering a larger volume with more radioactivity, we have investigated this route and the possibility of using it to circumvent the volume constraint we earlier experienced with pretargeting radiotherapy. Then, the bone marrow was flushed out with 1mL syringe. Targeting improves MSC treatment of inflammatory bowel disease. Clinical Ovarian and Other Gynecologic Cancer 5, 2426, doi: 10.1016/j.cloc.2011.08.001 (2012). Secondly, as mentioned above, there are plenty of immune cells in peritoneal cavity28, which are indispensable component of MSCs aggregates. The IP 'option' was not adopted, however, because the standard treatment had shifted to IV cisplatin plus . We have proposed that safe and effective protection against the development of adult onset cancers may be achieved by vaccination against tissue-specific self-proteins that are "retired" from expression at immunogenic levels in normal tissues as we age, but are overexpressed in emerging tumors. After 5 days of DSS contained water, all the subject mice showed strong positive test of fecal occult blood. Cancer Biother Radiopharm 28, 335342, doi: 10.1089/cbr.2012.1351 (2013). Interestingly, in some IP injection mice, GFP+ cells were observed in the epithelium of the inflamed colon. Arthritis and rheumatism 56, 11751186, doi: 10.1002/art.22511 (2007). Models should begin with local tissue infection, so that bacterial . The fluorescence imaging disclosed higher intensity of engrafted cells in inflamed colon and the corresponding mesentery lymph nodes (MLNs) in IP and AI groups than the IV group. Samples were prepared and tested as previously reported47. Epub 2020 Mar 18. 6B). Get the most important science stories of the day, free in your inbox. However, is IV injection the best way for MSCs delivery in IBD? Buy cheap Viagra Plus online no RX - Effective online Viagra Plus Study showed that genetically modified MSCs to increase CXCR-4 expression will lead to an increase of MSCs migration to intestinal with radiation enteritis and then resulted in disease improvement24. Peyrin-Biroulet, L., Loftus, E. V. Jr., Colombel, J. F. & Sandborn, W. J. PMC Cytokine in colonic protein extracts were determined by ELISA Kits according to the manufacturers instructions (R&D Systems, Minneapolis, MN). Intraperitoneal (IP) Injection (Injection of Sodium Pentobarbital .
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intraperitoneal injection in humans